PMC

Immunogold electron micrographs of resin-embedded mature green tomato pericarp showing polysaccharide epitopes in cell walls at primary pit fields and surrounding regions. A, The LM5 (1→4)-β-galactan epitope is absent from the cell wall at the pit field but is present throughout the rest of the primary cell wall (D). B, The JIM5 HG epitope is abundant throughout the pit field except at electron-translucent areas (*), which are likely to be callose deposits at the plasma membrane sides of the pit field. The HG epitope is also abundant throughout the primary cell wall (E). C, The LM6 (1→5)-α-arabinan epitope is not abundant in resin-embedded material but is detected at low levels in the cell wall at both pit fields (C) and the rest of the primary cell wall (F). Scale bars = 500 nm. c, Cytoplasm; v, vacuole; pf, pit field; ml, middle lamella.

Macro and microscopic observations. Figure 2A,2B,2C The resected intrapulmonary and mediastinal tumors(3.5 cm × 4.0 cm × 5 cm and 6 cm × 6 cm × 6 cm, respectively). Figure 2D Histological findings of the primary intrapulmonary tumor(PIT), H&E × 100. Figure 2E PIT H&E × 400. Figure 2F PIT CD99 Immunohistochemistry × 100. Figure 2G PIT CD99 Immunohistochemistry × 400. Figure 2H PIT CK 5& CK6 Immunohistochemistry × 100. Figure 2I PIT CK 5& CK6 Immunohistochemistry × 400. Figure 2J Histological findings of the primary mediastinal tumor(MT), H&E × 100. Figure 2K MT, H&E × 400.

A woman cleans an improved pit latrine in Guinea. Two-thirds of households in Guinea use pit latrines as their primary method of sanitation, and nearly that many rely on groundwater for their drinking water.
© Giacomo Pirozzi / Panos Pictures

Morphology of intervessel pits. (A) Thionin–methylene blue staining in light microscopy. F, fibres, P, pit, V, vessels. Scale bar = 20 µm. (B, C) PATAg staining in TEM. (B) Immature pit. (C) Mature pit. The membrane is highly labelled in the immature pit; the primary wall is strongly stained in both immature and mature pits. AA, annulus; PB, pit border; PM, pit membrane; PW, primary cell wall plus middle lamella; SW, secondary wall. Scale bars (B, C) = 1 µm.